GFP
PUBLICATIONS
357. “Simulation-guided engineering of GFPs with rapid β-strand dissociation and chromophore isomerization”, Yasmin B. Shamsudin, Alice R. Walker, Chey M. Jones, Todd J. Martínez & Steven G. Boxer, Nature Communications,14, 7401 (2023). [pdf]
355. “Optical control of ultrafast structural dynamics in a fluorescent protein”, Christopher D.M. Hutchison, James M. Baxter, Ann Fitzpatrick, Gabriel Dorlhiac, Alisia Fadini, Samuel Perrett, Karim Maghlaoui, Salomé Bodet Lefèvre, Violeta Cordon-Preciado, Josie L. Ferreira, Volha Chukhutsina, Douglas Garratt, Jonathan Barnard, Gediminas Galinis, Flo Glencross, Rhodri M. Morgan, Sian Stockton, Ben Taylor, Letong Yuan, Matthew G. Romei, Chi-Yun Lin, Jon P. Marangos, Marius Schmidt, Viktoria Chatrchyan, Tiago Buckup, Dmitriy Morozov, Jaehyun Park, Sehan Park, Intae Eom, Minseok Kim, Dogeun Jang, Hyeongi Choi, HyoJung Hyun, Gisu Park, Eriko Nango, Rie Tanaka, Shigeki Owada, Kensuke Tono, Takanori Nakane, Daniel P. DePonte, Sergio Carbajo, Matt Seaberg, Andy Aquila, Sebastien Boutet, Anton Barty, So Iwata, Steven G. Boxer, Gerrit Groenhof, Jasper J. van Thor, Nature Chemistry, 15, 1607-1615 (2023). [pdf]
354. “Structural Characterization of Fluorescent Proteins Using Tunable Femtosecond Stimulated Raman Spectroscopy”, Cheng Chen, Nathan J. Henderson, Dmitry A. Ruchkin, Jacob M. Kirsh, Mikhail S. Baranov, Alexey M. Bogdanov, Jeremy Mills, Steven G. Boxer, and Chong Fang, International Journal of Molecular Sciences, 24, 11991-12015 (2023). [pdf]
353. “Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism”, Jasper van Thor; Alisia Fadini; Christopher D.M. Hutchison; Dmitry Morozov; Jeffrey Chang; Karim Maghlaoui; Samuel Perrett; Fangjia Luo; Jeslyn C.X. Kho; Matthew G. Romei; Rhodri M. Morgan; Christian M. Orr; Violeta Cordon-Preciado; Takaaki Fujiwara; Nipawan Nuemket; Takehiko Tosha; Rie Tanaka; Shigeki Owada; Kensuke Tono; So Iwata; Steven G. Boxer; Gerrit Groenhof; Eriko Nango, Journal of the American Chemical Society, 145, 15796-15808 (2023). [pdf]
347. “Energetic basis and design of enzyme function demonstrated using GFP, an excited-state enzyme”, Chi-Yun Lin, Matthew G. Romei, Irimpan I. Mathews, Steven G. Boxer, Journal of the American Chemical Society, 144, 3968-3978 (2022). [pdf]
339. “Unusual Spectroscopic and Electric Field Sensitivity of Chromophores with Short Hydrogen Bonds: GFP and PYP as Model Systems”, Chi-Yun Lin and Steven G. Boxer, Journal of Physical Chemistry B, 124, 9513-9525 (2020). [pdf]
337. “Structural and spectroscopic characterization of photoactive yellow protein and photoswitchable fluorescent protein constructs containing heavy atoms”, Matthew G. Romei, Chi-Yun Lin, Steven G. Boxer, Journal of Photochemistry & Photobiology, A: Chemistry, 401, 112738 (2020). [pdf]
336. “Mechanism of color and photoacidity tuning for the protonated Green Fluorescent Protein chromophore,” Chi-Yun Lin and Steven G. Boxer, Journal of the American Chemical Society, 142, 11032-11041 (2020). [pdf]
331. “Electrostatic control of photoisomerization pathways in proteins”, Matthew G. Romei, Chi-Yun Lin, Irimpan I. Mathews, Steven G. Boxer, Science, 367, 76–79 (2020) [pdf] [Electrostatics affect the glow] [SLAC Story]
330. "Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins”, Jeffrey Chang, Matthew G. Romei, and Steven G. Boxer, Journal of the American Chemical Society, 141, 15504-15508 (2019) [pdf] [Jeffrey Chang in concert]
329. "Unified Model for Photophysical and Electro-Optical Properties of Green Fluorescent Proteins", Chi-Yun Lin, Matthew G. Romei, Luke M. Oltrogge, Irimpan I. Mathews, and Steven G. Boxer, Journal of the American Chemical Society, 141, 15250-15265 (2019) [pdf]
326. “Split Green Fluorescent Proteins (GFPs): Scope, Limitations, and Outlook”, Matthew G. Romei and Steven G. Boxer, Annual Reviews of Biophysics, 48, 19-44 (2019) [pdf]
319. "Structural Insight into the Photochemistry of Split Green Fluorescent Proteins: A Unique Role for a His-tag", Alan Deng and Steven G. Boxer, Journal of the American Chemical Society, 140, 375–381(2018). [pdf]
314. "Mechanism and bottlenecks in strand photodissociation of split green fluorescent proteins (GFPs)", Chi-Yun Lin (林其勻), Johan Both, Keunbong Do, and Steven G. Boxer, PNAS, Published online, E2146–E2155 (2017). [pdf]
306. "Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP)", Luke M. Oltrogge and Steven G. Boxer, ACS Central Science, 1 , 148-156 (2015). [pdf] [Commentary]
302. “Ground-state Proton Transfer Kinetics in Green Fluorescent Protein (GFP)”, Luke M. Oltrogge, Quan Wang, and Steven G. Boxer, Biochemistry, 53, 5947-5957 (2014). [pdf]
296. “GFP Variants with Alternative β‑Strands and Their Application as Light-driven Protease Sensors: A Tale of Two Tails”, Keunbong Do and Steven G. Boxer, Journal of the American Chemical Society, 135 , 10226-10229 (2013). [pdf]
280. "Thermodynamics, Kinetics, and Photochemistry of Beta-Strand Association and Dissociation in a Split-GFP System", Keunbong Do and Steven G. Boxer, J. Am. Chem. Soc. 133, 18078-18081 (2011). [pdf] [Supplement]
273. "Light-Activated Reassembly of Split Green Fluorescent Protein", Kevin P. Kent and Steven G. Boxer, J. Am. Chem. Soc., 133, 4046-4052(2011). [pdf] [Supplement]
267. "Synthetic Control of Green Fluorescent Protein", Kevin P. Kent, Luke M. Oltrogge, Steven G. Boxer, Journal of the American Chemical Society, 131, 15988-15989, (2009). [pdf] [Supplement] [C&E News]
259."Deconstructing Green Fluorescent Protein", Kevin Kent, William Childs, and Steven G. Boxer, J. Am. Chem. Soc., 130, 9664-9665, (2008). [pdf] [Supplement]
254. "Dynamic Stokes Shift in Green Fluorescent Protein Variants", Paul Abbyad, William Childs, Xinghua Shi, and Steven G. Boxer, PNAS, 104, 51, 20189-20194. (2007). [pdf]
253. "Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C): Quantitative Analysis of FRET in YFP Dimers", Xinghua Shi, Jaswir Basran, Harriet E. Seward, William Childs, Clive R. Bagshaw, and Steven G. Boxer, Biochemistry, 46, 14403-14417. (2007). [pdf]
250. "Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D. 2. Unusual Photophysical Properties", Xinghua Shi, Paul Abbyad, Xiaokun Shu, Karen Kallio, Pakorn Kanchanawong, William Childs, S. James Remington, and Steven G. Boxer, Biochemistry, 46, 12014-12025 (2007). [pdf]
249. "Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D. 1. Mutagenesis and Structural Studies", Xiaokun Shu, Karen Kallio, Xinghua Shi, Paul Abbyad, Pakorn Kanchanawong, William Childs, Steven G. Boxer, and S. James Remington, Biochemistry, 46, 12005-12013 (2007). [pdf] [Supplement]
232."Enhancement of the Fluorescence of the Blue Fluorescent Proteins by High Pressure or Low Temperature", Koit Mauring, Jason Deich, Federico I. Rosell, Tim B. McAnaney, W.E. Moerner, and Steven G. Boxer, J. Phys. Chem. B, 109, 12976-12981 (2005). [pdf]
231."Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors. 3. Temperature Dependence of Proton Transfer", Tim B. McAnaney, Xinghua Shi, Paul Abbyad, Henry Jung, S. James Remington, and Steven G. Boxer, Biochemistry, 44, 8701-8711 (2005). [pdf]
229."Protonation, Photobleaching, and Photoactivation of Yellow Fluorescent Protein (YFP 10C): A Unifying Mechanism", Tim B. McAnaney, Wei Zeng, Camille F. E. Doe, Nina Bhanji, Stuart Wakelin, David S. Pearson, Paul Abbyad, Xinghua Shi, Steven G. Boxer, and Clive R. Bagshaw, Biochemistry, 44, 5510-5524 (2005). [pdf]
215."Polarized Absorption Spectra of Green Fluorescent Protein Single Crystals: Transition Dipole Moment Directions", Federico I. Rosell and Steven G. Boxer, Biochemistry, 42, 177-183 (2003) . [pdf]
212."Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors. 2. Excited-State Dynamics", Tim McAnaney, Eun Sun Park, George T. Hanson, Steven G. Boxer, and S. James Remington, Biochemistry, 41, 15489-15494 (2002). [pdf]
211."Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors. 1. Structural Characterization and Preliminary Application", George T. Hanson, Tim McAnaney, Eun Sun Park, Marla E. P. Rendell, Daniel K. Yarbrough, Shaoyou Chu, Lixuan Xi, Steven G. Boxer, Marshall H. Montrose, and S. James Remington, Biochemistry, 41, 15477-15488 (2002). [pdf]
196. "Photophysics of DsRed, a Red Fluorescent Protein, from the Ensemble to the Single-Molecule Level", B. Lounis, J. Deich, F. I. Rosell, Steven G. Boxer, and W. E. Moerner, J. Phys. Chem. B, 105, 5048-5054 (2001). [pdf]
163. "The Electronic Structure of the Chromophore in Green Fluorescent Protein (GFP)". Gerold Bublitz, Brett King, and Steven G. Boxer, Journal of the American Chemical Society, 36, 9370-9371 (1998). [pdf]
151. "Crystal Structure and Photodynamic Behavior of the Blue-emission Variant Y66H/Y145F of Green Fluorescent Protein", Rebekka M. Wachter, Brett A. King, Roger Heim, Karen Kallio, Roger Y. Tsien, Steven G. Boxer, and S. James Remington, Biochemistry, 36, 9759-9765 (1997). [pdf]
138. "Another Green Revolution", Steven G. Boxer, Nature News & Views, 383, 484-485 (1996). [pdf]
136. "Ultra-fast Excited State Dynamics in Green Fluorescent Protein: Multiple States and Proton Transfer", Mita Chattoraj, Brett A. King, Gerold U. Bublitz, and Steven G. Boxer, Proceedings of the National Academy of Sciences, 93, 8362-8367 (1996). [pdf]